Composite

Part:BBa_K2688023:Design

Designed by: William Briand   Group: iGEM18_GO_Paris-Saclay   (2018-09-23)


tetO_RBS_ler2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene ler2 encodes the Ler protein of E. coli E2348/69 (NCBI Reference Sequence: WP_001253284.1). Its nucleotide sequence is naturally devoid of Biobrick forbidden sites.


Source

The gene ler2 was amplified by PCR from a lab collection plasmid of Leh and collaborators (Leh et al. mBIO, 2017) and cloned within pSB1C3 with its native RBS.

References